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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 10 Documents

Search results for , issue "Vol 3, No 1 (2019): Biology" : 10 Documents clear

Morphological Indices of Drought Tolerant of Some Paddy Varieties (Oryza sativa L.) In West Sumatera Using Standard Evaluation System (SES) For Rice syauli mardita; Violita Violita
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Rice is an important food crop that consumed by more than half of the world's population, but almost every year there is decrease rice production in field, this is due availability of land and water as main source of agriculture. Therefore optimization of dry land as agricultural is needed, but many things are of concern to dry land, especially water sources are minimal. Therefore, it is need have rice seeds are suitable and adaptive to dry land, the response of rice plants to drought can observed from physiological, morphological and anatomical changes. This research was conducted in August to December 2018 in research laboratories, wire houses and plant physiology laboratories, Faculty of Mathematics and Natural Sciences, Padang State University. This study was to obtain a drought tolerant morphological index from several paddy seeds in West Sumatera, so indexes showed that rice seeds were tolerant to drought by observing morphological changes, namely leaf rolling (DPD), shoot top index (IKP). ), and plant recorvry (TP) using the SES (Standard Evaluation System) method for rice. Data is processed using cluster analysis with hierarchical analysis methods. The results showed the most resistant varieties to drought stress were’s Baroto, Situ Bagendit, Randah Kuniang, and Harum.

The Effect Of Time Differences On Polyacrilamide Gel Electrophoresis On Cassava DNA Nur Ayu Ramadanti; Dwi Hilda Putri
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

DNA bands formed from the results of electrophoresis with Polyacrilamide gel are considered as 1 character representing 1 allele. PCR products produce multiple bands (multy bands), which indicates that there are multi alleles in the sample. Electrophoresis is a chemical analysis method based on the movement of charged protein molecules in the electric field. Separation is carried out based on differences in the size of the molecular weight and the electric charge contained by the macro-molecule. In addition, the effect of gel concentratio n, buffer and electrophoresis time also has a role in the results of electrophoresis. This study was conducted to compare the best separation time for acrilamide gel electrophoresis with the results of cassava DNA amplification. The materials used in this study are two cassava varieties, namely: Adira IV 1, Adira IV 2, Adira IV 3, Carvita 25 1, Carvita 25 2, and Carvita 25 3. Using electrophoresis by poly-acrilamide gel with two different time effects: 1 hour 30 minutes and 3 hours. The results of electrophoresis with 3 hours gave better results of DNA visualization compared to 1 hour 30 minutes.

Comparison of Three Different DNA Isolation Methods To Degradate The Trichoderma Fungi Cell Wall Widya Ruchi; Dwi Hilda Putri; Azwir Anhar
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Dinding sel jamur Trichoderma tersusun atas senyawa kitin yang sangat kokoh dan resisten terhadap aktivitas enzim. Teknik dan metode spesifik diperlukan untuk mendegradasi dinding sel jamur. Penelitian ini bertujuan untuk menganalisis metode isolasi DNA terbaik dalam melisiskan dinding sel jamur dengan membandingkan beberapa metode isolasi. Metode isolasi yang digunakan adalah DNeasy Plant Mini Kit, kombinasi DNeasy Plant Mini Kit dan pemanasan, serta kombinasi DNeasy Plant Mini Kit dan cara fisik (menggerus sampel dengan menggunakan mortar dan pestle dalam nitrogen cair). Penelitian ini dilakukan pada bulan Maret sampai September 2018 yang bertempat di Laboratorium Mikrobiologi, Laboratorium Bioteknologi dan Genetika, serta Laboratorium Penelitian Terpadu, FMIPA, UNP. Data dianalisis secara kualitatif dengan elektroforesis gel agarose dan secara kuantitatif dengan menghitung nilai kemurnian dan konsentrasi DNA menggunakan Nanodrop. Hasil penelitian menunjukkan bahwa metode kombinasi DNeasy Plant Mini Kit dengan cara fisik memberikan hasil isolasi DNA dengan konsentrasi yang tertinggi yaitu 2,4 μg/ml dengan nilai kemurnian 1,857. Pada metode DNeasy Plant Mini Kit dan metode kombinasi DNeasy Plant Mini Kit dengan pemanasan, konsentrasi DNA yang diperoleh berturut-turut 1,211 µg/ml dan 0,933 µg/ml dengan nilai kemurnian 1,728 dan 1,708. Hasil uji elektroforesis menunjukkan adanya pita DNA berupa garis tipis pada sampel yang diisolasi dengan kombinasi metode kit dan cara fisik, sedangkan pada kedua sampel lainnya tidak ditemukan adanya pita DNA. Dapat disimpulkan metode isolasi DNA yang dapat dijadikan rujukan untuk mendegradasi dinding sel jamur Trichoderma adalah metode kombinasi kit dengan nitrogen cair dengan catatan dibutuhkan penambahan kadar isolat yang digunakan.Kata Kunci: Isolasi DNA, Dinding sel, Trichoderma

The Effect of N-Hexan Extract of Sambiloto Leaf (Andrographis paniculata) on Reproduction Function of Female ICR of Mice (Mus musculus) Period of Praimplantation and Pascaimplantation Muliana Muliana; Sukmawati Sukmawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract. The objective of this research is to know the effect of giving of n-hexan extract of A. Paniculata leaf on the reproduction function on female mice and when hypodermic of how many dose of extract n-hexan most having an effect on the reproduction function on female mice. Extract gift conducted by inoculation. Mice or test animal obtained from Hall of Animal Research (BPH) of Regency Maros. Old age the mencit 10-12 week of weighing of mean body 21g, while test substance used by leaf of Andrographis paniculata obtained in regency of Bone of subdistrict of Sibulue of countryside of Pattiro Bajo. Parameter perceived that is s the number of implantation, number of life fetus, gestation loss, dead foetus, embryo resorbsi, corpus luteum, and death pascaimplantasi. This Research represent the research of experiment and desain research used that is Complete Random Device ( RAL) consisted of by three treatment group five times is restating so that obtained by fifteen combination. Result of research indicate that the gift of n-hexan extract of A. Paniculata leaf at dose level tested degrade the number of implantation, number of life fetus, improving number of gestation loss, and number of died fetus , also generate the effect fitotoksik of body weight of mice mains. From inferential research result that extract of n-hexan A. Paniculata leaf can effect on the reproduction function on female mice with the hypodermic time most having an in with day of pregnancy to-0 until pregnancy day to-4.

Histopathological Changes of Testes and Testosterone Level of Mice that are Exposed to Permot Leaf Mosquito Mat (Passiflora foetida) Rina Priastini Susilowati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

In this study, mice testicular degeneration was influenced by exposure to the mosquito mat made from transfluthrin 3000 ppm and permot leaf mosquito mat which were evaluated based on observations on testicular histopathological changes and testosterone level. Fifteen Balb C male mice aged 2-3 months with a body weight of 25-30 g were divided into five groups, namely A, B, C, D, and E, with each group consisting of 3 replications. Group A was the negative control group (without exposure), group B was the exposure group for the mosquito mat made from transfluthrin 3000 ppm, group C, D, and E were the exposure groups of mosquito mat with permot leaves doses of 1000 ppm, 2000 ppm and 3000 ppm. Each exposure group was given treatment 8 hours per day from 18.00 - 04.00 for 3 months. The results showed a significant decrease in testicular weight between the control group and the treatment group. There were also significant differences in the testosterone level observed in the treatment group, as well as observations on the spermatogenic index (IS). For histopathological observation of testicular mice, there was a reduction in epithelial cell spermatogenic and diameter of seminiferous tubule. The results of the Kruskall Wallis test showed a significant difference between the control group and the treatment group of the mosquito mat made from transflutrin 3000 ppm. The conclusion is that exposure to mosquito mat made of permot peaves up to a 3000 ppm dose is safe to use and can kill the Aedes aegypti mosquito effectively.

Dimensions of Fiber and Jabon Wood Fiber Derivative Value (Anthocephalus cadamba [Roxb] Miq.) in Sialang Dharmasraya and Tabing Padang Regions Adillah Syafitri; Vauzia Vauzia; Des M
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0201931102805-0-00

Jabon (Anthocephalus cadamba [Roxb] Miq.) Is a type of wood that is fast growing and has adaptability in various habitat types. Anatomical characteristics of wood are strongly influenced by environmental factors. This study looked at the dimensions of fiber and the derivative value of jabon wood fiber from the Sialang Dharmasraya and Tabing Padang regions. This research is a descriptive research. Observation of fiber dimensions using wood maceration techniques following the Forest Products Laboratory Method. The results showed that the value of wood fiber dimensions from the Sialang area was higher than those from the Tabing area. In Sialang it has wood fiber length of 603.82-1061.23µm, fiber diameter of 12.37-21.22µm, lumen diameter of 3.81-12.97µm, and wall thickness of 2.75-7.49µm. Whereas, in the Tabing area it has wood fiber length of 592.91-844.38µm, fiber diameter 11.80-20.35µm, lumen diameter 6.14-14.35m, and wall thickness of 1.91-4.54µm. The derivative value of jabon Tabing wood fiber is higher (runkel ratio 0.63-0.62, felting power 41.49-50.25, flexibility ratio 0.70-0.52, coefficient of rigidity 0.22-0.16 and mulsteph ratio 50.27-72.92%) compared to Sialang area (runkel ratio 1.15-1.44, felting power 50.01-48.81, flexibility ratio 0.61-0.30, coefficient of rigidity 0.35-0.22, and mulsteph ratio 62.64 to 90.51%). The quality value of jabon wood fiber at Tabing is better than Sialang. The results of this study can be used as information in jabon wood cultivation.

Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate Nada Nafion; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Cases of bacterial resistance to antibiotics are discussed with serious problems in the world of health. New antimicrobial compounds are needed which are more effective in treating infectious diseases. Isolate B.J.T.A-6 is an endophytic bacteria from Andalas plants (Morus macroura Miq.) which is known to be able to produce antimicrobial active compounds. Antimicrobial compounds can be produced by growing them on fermented media. The purpose of this study was to optimize the medium fermentation of Andalas endophytic bacteria of B.J.T.A-6 isolates in producing antimicrobial compounds. While medium fermentation is Nutrient Broth (NB), Muller Hinton (MH), and Luria Bertani Broth (LB). Antimicrobial activity tests were carried out by means of diffusion. The parameters used were the diameter of the inhibition zone against Staphylococcus aureus around the disc paper. The profile of medium fermentation optimization was analyzed statistically. The results showed that B.J.T.A-6 isolates could inhibit the highest growth of S. aureus by using LB fermentation medium.

Potential of Yields and Starch Production from Several Local Cassava Genotypes Hartati - Hartati; N Sri Hartati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The need for cassava starch is increasing along with its aplication in industries. Our study aim to determine the yield and production of starch from several local cassava genotypes. Forty-one cassava genotypes from LIPI collection were used to produce starch. Cassava tubers were harvested from five trees of each genotype. The number and the weight of tuber from each genotypes were then calculated. Around 2500 grams of tuber from each genotypes were grated and extracted to produce the starch. The results showed that cassava with the highest yield was Kristal Merah (15,460 Kg), and followed by PNG1 (15,1Kg), Menti (9,970Kg) and Roti (9,380Kg). The genotype with the highest tuber weight is PNG1, and then followed by Roti , Menti and Kristal Merah. Eleven genotypes of cassava produced starch with levels above 25%. Based on our results, the genotypes that potentially used as raw material for the starch industry were Kristal Merah, PNG, Menti and Roti.Keywords: cassava, genotypes, starch, tuber’s weight, yield

Effect of Boiled Water Tithonia diversifolia A. Gray Leaf Against the Pancreas Histology in Mus musculus L. Induced by Alloxan Yenni Fitri; Elsa Yuniarti
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Traditional medicine is one of the drugs used by the community to be one of the efforts to treat diseases. One of them is Diabetes Mellitus (DM). DM is a metabolic disease characterized by hyperglycemia which results in an increase in free radicals in the cell. DM treatment is quite expensive so an alternative drug is needed. One of them is Tithonia diversifolia A. Gray. This study used a completely randomized design, consisting of 1 control, 4 treatments (P1: Only alloxan induced), P2: Alloxan 65 mg / kg BB, P3: Metformin 65 mg / kg BB, P4: Boiled leaves of moon leaves 24.6 mg / 10 ml and P4: Moon flower leaves boiled water 49.1 mg / 10 ml. The parameters observed were blood sugar in male mice and the number of cells in the islands of Langerhans before and after being given boiled leaves of moon flowers and metformin. Data were analyzed using ANOVA then continued with DMRT test with a significant difference of 5%. The results showed that administration of boiled kembang bulan leaves and metformin for 7 days could reduce blood sugar levels in mice and accelerate the regeneration of pancreatic cells. The most significant impact is the treatment with the highest dose, namely P4 (Moon leaf leaves boiled water 49.1 mg / 10 ml).

The Influence of Bioinsektiidide Variation of Tuba Root Extract (Derris elliptica Roxb. Benth) On Phantsahm Mortality the Pest (Leptocorisa Acuta Thumberg) I Gede Warse; Handoko Santoso; Rasuane Noor
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0201931103757-0-00

Leptocorisa acuta Thumberg is one of the pests that many rice farmers complain about. This pest can cause damage to rice plants while still young, so the results obtained are not optimal. Based on these problems it is necessary to use insecticides so that the pest can be controlled. Derris elliptica Roxb. Benth is one of the many plants that grow in Indonesia. The root of this plant has a chemical compound in the form of rotenone which has the potential as a bioinsecticide to eradicate pest parasites. The study was an experimental study using a completely randomized design method with three treatments and one control with 6 replications. The treatment dose in this study using root extract Derris elliptica Roxb. Benth. Bent: 8%, 10%, 12% and 0% control. Based on the Kruskal Wallis Test the results were x2calculate 10.88 > x2table 9.49 at a 0.05 in the Chi-square table, so that there was an effect of the variation of the bioinsecticide dose of root extract Derris elliptica Roxb. Benth the impact on mortality of the Imago Leptocorisa acuta Thumberg. Based on the average ranking of treatment at a dose of 12%: 6.7, a dose of 10%: 8.46, a dose of 12%: 8.76 and a control of 0%: 18, so it can be concluded that root extract Derris elliptica Roxb. Benth the most effective against the mortality of imago Leptocorisa acuta Thumberg was at a dose of 12%.

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